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Scientific, Technological and Production Journal
ISSN 2079–3332 (print)
ISSN 3034-6835 (online)
founded in 2001

Optimization of nutrient medium for cultivation in a shaker incubator of an autochthonous mixed culture isolated from oxidized ore of the Shanuch deposit

Original articles: 2024 No 5

Optimization of nutrient medium for cultivation in a shaker incubator of an autochthonous mixed culture isolated from oxidized ore of the Shanuch deposit

DOI: 10.56195/20793332_2024_5_55_59

Read in pdf S. O. Ocheretyana
  1. Research Geotechnological Center of the Far Eastern Branch of the Russian Academy of Sciences, Petropavlovsk-Kamchatsky, Russia
p. 55-59, No.5/2024
Abstract:
The article presents the results of experiments investigating the proportions of mixing ore with bacterial solutions that can be useful in the extraction of metals in hydrometallurgy. The degree of inoculum dilution on the growth of the autochthonous mixed bacterial culture isolated from the oxidized ore of the Shanuch deposit is analyzed. The experiments were carried out in two loading options: inoculum + ore and bacterial suspension + ore and at different solid to liquid ratios of 1:5 and 1:8. The results showed that an increase in the dilution degree (1:8) leads to a more significant cell growth at the later stages of the experiment. For example, with the bacterial suspension + ore (1:8) option, the cell count increased sharply to 133.4 × 107 cells/ml. In general, the experiments confirm that dilution promotes the growth of bacteria at the later stages, which may be important for the optimization of bacterial-chemical technologies for processing sulfide ores.
Keywords:
acidophiles, chemolithotrophs, bacteria, Shanuch deposit, nutrient media, biomass, planktonic forms, bioleaching, bacterial-chemical leaching, biohydrometallurgy
For citation:
Ocheretyana S. O. Optimization of nutrient medium for cultivation in a shaker incubator of an autochthonous mixed culture isolated from oxidized ore of the Shanuch deposit. Mine Surveying and Subsurface Use. 2024;(5):55-59. (In Russ.). https://doi.or g/10.56195/20793332_2024_5_55_59.
Information about the authors:
  1. Svetlana O. Ocheretyana – Cand. Sci. (Biol.), Senior Researcher, Research Geotechnological Center Far Eastern Branch of the Russian Academy of Sciences (NIGTC FEB RAS), Petropavlovsk-Kamchatsky, Russian Federation, https://orcid.org/0009-0008-6221-8239, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
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